This glossary defines the column headings used in the 2DWG meta-database.
- Tissue or Organelle
- Tissue or organelle of origin of sample. If there are
subcategories, they are appended to the right (eg. lymphocyte-T,
etc.)
- Species
- Species of the sample.
- Cell line (strain)
- Cell line or strain of sample if applicable.
- Image URL
- URL to the gel image. Currently ftp:// and http:// protocols
and GIF, JPEG or TIFF images are accepted.
- DB URL
- URL to the specific database where this image resides and which may
discuss this data in more detail. This database may also include
spot maps of identified proteins.
- Isotope / stain / Ab
- Detection method. Typically isotope implies autoradiographs or
phosphor-imaging (in which case the radioisotope is mentioned,
eg. 35[S]-met); stain (eg. Coomassie Blue or silver for
silver stain); and Ab is antibody (eg. anti-PY, anti-p53, etc.).
- CA/IPG
- First dimension method: Carrier Ampholytes or Immobilized pH
Gradients.
- IEF/NEPHGE
- Type of gel: IsoElectric Focusing or Non-Equilibrium PH Gradient
Electrophoresis.
- pH range
- The isoelectric pH range if known. If the range is specified as
8-4, then this means that acid is on the right, the default is acid is
on the left (eg 4-8).
- Mr (Kd) range
- The Molecular Mass
range if known. If the range is specified with "mwm", this indicates
that molecular weight markers are used to specify the range.
- Lab/ Org/ Comp
- The name of the Laboratory, Organization or
Company where the web database resides. This entry is linked to
thetop level web page for that organization.
- Scan/synth/diagrm
- Whether the image is a scan of a single gel, a
composite of a number of gels, a synthetic gel, or a
diagram of spot positions.
- Map/raw data
- Whether the image is a raw gel image or a spot map (with
proteins identified by name or number indices used by the particular
DB scheme). If a URL is available for the spot map, then it is
provided. Note that for some databases although a map exists, you must
track down the spot mapping of numbers to protein identifications in
associated papers. In other databases, there are active gel images
where the user can click on an image to look up the database contents
(if any) for that spot.
- Miscellaneous
- Additional information about the gel, sample, etc. This should
complement the other fields so the entry is sufficient to identify the
material.
- 2DWG ID #
- Identification number WG00001, WG00002, ... for the 2DWG database
assigned by the 2DWG during the
submission process.
$Date: 1997/10/30 19:40:03 $ /
lemkin@ncifcrf.gov
(P. Lemkin, LECB, NCI)