Limitations with the Flicker Method
- Similar data - comparisons are only potentially as good as
data being compared:
- Use similar material
- Prepare gels under somewhat similar conditions
- Spot identification - comparisons will only give
putative identifications
but may suggest antibodies to try without having
to sequence spots of interest
- pIe, MW, OD Calibration - current design doesn't
calibrate 2D gels in terms of pIe, molecular mass, and
optical density (OD)
- Some users occasionally have problems visualizing
changes or are bothered by flickering images
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A
DEMO: Demo