Example - Comparing 2D PAGE Gels May Suggest
Putative Spot Identification
- Scientists around the world often work on similar 2D gel data
- Many 2D gel image Web
databases provide protein identification spot maps
- Comparing samples against maps may suggest putative protein
spot identification
- How can we do this comparison? Through visual comparison - to be discussed
Corresponding regions of plasma protein gels from different labs
SWISS-PROT/2DPAGE 2D gel Database Merril (NIMH)
Immobilized pH Gradient Carrier Ampholytes
Non-linear gradient Linear Gradient
http://www.expasy.ch/
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