Select Pair of Images to Flicker Compare from 2D DNA gel study
This subset of gels was from a study of 2-D DNA
alterations in K-ras trasformed NIH-3T3 cell lines in the Laboratory
of Dr. Ming You, at the Medical College of Ohio. Samples include four
K-ras transform NIH-3T3 2-D images, and three control NIH-3T3 images.
The References describe the preparation and
experimental conditions of this study.
Overview of 2D DNA gel Study
In this study, a two-dimensional gel electrophoresis (2-DGE) assay was
used to scan the entire genome of K-ras trasformed NIH-3T3 cell lines
for DNA alterations. This method involves cleaving of high molecular
weight DNA by three restriction enzymes (Not I, EcoR V, and Hind III),
radioactive labeling and separating DNA fragments by 2-DGE. The
labeled fragments generate thousands of individual spots on X-ray
films. The radioactive intensity of each spot represents the copy
number of this fragment in the genome. By comparing the 2-DGE profile
of transformed cell lines with their normal counterparts, genetic
alterations such as amplification, and allelic loss can be
detected.
For more information about this study and other 2D DNA experiments
contact
James L. Patrick or Dr. Ming You, Department of Pathology,
Medical College of Ohio Toledo, Ohio. 419-381-3455 jpatrick@opus.mco.edu
(J. Patrick) /
myou@gemini.mco.edu (M. You)
Hint: suggested comparison of control-1 with K-ras2. Set the control-1
central image position to (115,170) and that of K-ras2 to (123,174).
This differents represents a prime candidate spot of DNA to cut out,
clone and amplify with PCR. It has a p-value of 0.001.
1. First select a different image for the Left and Right
images. This entails a) setting the radio button for the source type
and b) selecting the particular image from the scrollable list or
typing it directly. You may use any of the three from image sources
available for both the Left and Right images. Note: you can only
compare different images (not an image against itself).
2. Then press Go Flicker to generate a Flicker comparison of
this pair of images.
3. Please while
images load. Depending on the access speed of the web site where
the images reside, this may take several minutes.
Flicker Image Comparison: Flicker is a Java program for
comparing two images. See the Flicker home page for
more information on Flicker.
- Patrick JL, (1997) Analysis Of Restriction Landmark Genomic
Scanning Images Of DNA From K-Ras Oncogene Transformed NIH3T3 Cells
Using
GELLAB II+ Software. Proc Am Assoc Cancer Res.39:1098
- Liu J, (1997) Detection of Genomic Alterations in Human Cervical
Cancer by Two-Dimensional Gel Electrophoresis J. Cellular
Biochemistry. 64: 2
- Liu J, (1995) Detection of genetic alterations in rat mammary
tumors by two-dimensional gel electrophoresis. Proc Am Assoc
Cancer Res. 36: 543
-
Nagai H, (1994) Aberration of genomic DNA is association with
human hepatocellular carcinomas detected by 2-dimensional gel
analysis. Cancer Res 54:1545-1550,
-
Hayashizaki Y, (1993)Restriction landmark genomic scanning method and its various applications.
Electrophoresis 14:251-258
-
Hirotsune S, (1992) New approach for detection of amplification in cancer DNA using restriction landmark genomic scanning. Cancer Res.52:3642-3647
-
Hatada I, (1991) A genomic scanning method for higher organisms using restriction sites as landmarks. Proc Natl Acad Sci 88:9523-9527
- Rogan P, Lemkin PF, Klar A, Singh J, Strathern J (1991)
Two-dimensional agarose gel electrophoresis of restriction-digested
genomic DNA. Methods: A Companion to Methods Enzymol.
3(2), 91-97.
Each of these controls and K-ras gels represent different replicate
cell lines to demonstrate the reproducibility of this assay.
Back to the Flicker Home Page
$Date: 1998/12/07 14:18:23 $
jpatrick@opus.mco.edu
(J. Patrick), Medical College of Ohio /
lemkin@ncifcrf.gov,
(LECB,NCI/FCRDC)
