Select a Pair of Breast Cancer 2D Gel Images to Flicker Compare

These breast cancer gels are from several laboratories. These include the NCI drug discovery program (see Weinstein, NCI/Bethesda (1)), and the breast cancer study ( Giometti, PMG/Argonne (2,3)).

The MCF7-0001-L gel images represent the central portions of gels from MCF7 cell line derivatives selected for drug resistance by exposure to various anticancer drugs. The variants are denoted L621B, L612C, L612E, L612J, L612L, L612N, L612P, L612Q, L612S, and L614P, L614Q and L614S. (See discussion in the Breast Cancer Drug Screen Flicker page for more details).

Two human breast N537 samples which contrast differences between the the MCF7 and MB231 cell lines (Giometti, Argonne Labs).

These gels are also found in the 2DWG meta-database where you can get a breast gel subset of the 2DWG. We provide a list of the individual gels here to make it easier to review them.

A protein map of some of the proteins in these gels is maintained by J. Weinstein). See also Protein Expression Database.

To Flicker Compare two gels:

Flicker Image Comparison: Flicker is a Java program for comparing two images. See the Flicker home page for more information on Flicker.

1. First select a different image for the Left and Right images. This entails a) setting the radio button for the source type and b) selecting the particular image from the scrollable list or typing it directly. You may use any of the three from image sources available for both the Left and Right images. Note: you can only compare different images (not an image against itself).

2. Then press Go Flicker to generate a Flicker comparison of this pair of images.

3. Please WAIT while images load. Depending on the access speed of the web site where the images reside, this may take several minutes.

Canvas Size,
Left Image:
NCI Flicker Server image (left)


Right Image:
NCI Flicker Server image (right)

Enter any image URL (http:// or ftp://) (right)


List of human breast gel images


References

  1. Weinstein JN, Myers TG, O'Connor PM, Friend SH, Fornace AJ Jr, Kohn KW, Fojo T, Bates SE, Rubinstein LV, Anderson NL, Buolamwini JK, van Osdol WW, Monks AP, Scudiero DA, Sausville EA, Zaharevitz DW, Bunow B, Viswanadhan VN, Johnson GS, Wittes RE, Paull KD (1997). An information-intensive approach to the molecular pharmacology of cancer. Science 275 343-349.

  2. Giometti CS, Tollaksen SL, Chubb C, Williams C, Huberman E (1995) Analysis of proteins from human breast epithelial cells using two-dimensional electrophoresis Electrophoresis 16, 1215-1224.

  3. Giometti, CS, Tollaksen, SL, Williams, K (1997) A 2-DE database of human breast epithelial cell proteins Electrophoresis, in press.
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$Date: 1999/09/09 20:58:31 $ /
csgiometti@anl.gov (Giometti)
weinstein@dtpax2.ncifcrf.gov (Weinstein)
lemkin@ncifcrf.gov (Lemkin, Flicker), (LECB,NCI/FCRDC)