Flicker compare protein phosphorylation 2D studies in mouse B/T lymphocytes

This data is taken from the phosphoDB Phosphoprotein database which identifies some of the proteins in these gels. The gels are murine B lymphoma cells (WEHI-231) labeled with 32P-orthophosphate, treated with PMA (50 ng/ml) for 12 min, and lysed with NP-40 as previously described (J Biol Chem 261, 14817). The left gel was treated with PMA and the second was not. The first gel may be used with "Click to access DB" in Flicker.

See the Flicker Reference Manual for more details on running flicker. You can get data from that database from flicker if you 1) set the "Click to access DB" checkbox, and 2) click on a spot.

To navigate around an image, you can use the scroll bars and then click on the spot of interest. Hint: however, it is easier if you hold the CONTROL key and then click on a point in the image. This will center that point in the scrollable window. It will also display the (x,y) position of the cursor in that image at the top of that window. After you have done an initial alignment, you can move both images "ganged" together by clicking on a spot in either image with both the SHIFT and CONTROL keys pressed.

Click on the Flicker checkbox to turn flickering on or off. You align the images by navigating one of the two as in the previous step until you are satisfied with the alignment.

Note that only the 34g, B lymphoma cells (WEHI-231), gel is active with the "Click to access DB" option. Clicking on a spot with the checkbox set will search the phosphoDB for data on that spot.

Left Image: 34g - B lymphoma cells (WEHI-231) treated with PMA
Right Image: Blymphoma - B lymphoma cells (WEHI-231) not treated with PMA

Please WAIT while images load.

$Date: 1998/12/01 14:30:14 $ lemkin@ncifcrf.gov