Create pairs of images to Flicker Compare from protein phosphorylation studies in mouse T lymphocytes 








Introduction

Three sets of images of murine T cell phosphoproteins were chosen that 
illustrate: 1) alterations in phosphorylation during the cell cycle, 2) 
the differential regulation of tyrosine-phosphorylation in cells grown 
in IL2 or IL4, and 3) the differential regulation of 32P-labelled
proteins in cells grown in IL2 or IL4.



I.  Cell-cycle specific phosphorylation
 

The first pair of images demonstrate differences in protein
phosphorylation at different stages of the cell cycle. 
CT.4R cells were labeled with 32P-orthophosphate prior to lysis
and analysis as 
previously described.  The first image (59g-PPDB-G1-phase)
represents proteins from cells enriched in the G1-phase of the cell
cycle, while the second image (59h-PPDB-G2/M phase) represents cells
in late G2 and M.



II.  Tyrosine phosphorylation during growth in IL2 and IL4


The second pair of images are of CT.4R cells grown in IL4 or IL2.
Tyrosine-phosphorylated proteins were visualized by Western blotting.
The first image (68dpy-PPDB-IL4) is derived from cells grown in IL4
and the second image (68epy-PPDB-IL2) from cells grown in IL2.



III.  Total protein phosphorylation during growth in IL2 and IL4
  

The third pair of images are of CT.4R cells grown in IL4 or IL2. Cells
were labeled with 32P-orthophosphate prior to analysis.  The first
image (68dpy-PPDB-IL4) is derived from cells grown in IL4 and the
second image (68epy-PPDB-IL2) is from cells grown in IL2.



 

These 2D PAGE gel images are from a larger study of the regulation of
protein phosphoprotein by IL2 and IL4 in murine T cells that was
initially supported by the American Cancer Society and the Arthritis
Foundation. A  phosphoprotein database of
some of the proteins identified in these gels is under construction.
The six images of phosphoproteins linked to in this page are Copyright
© 1997 by P.V. Hornbeck, All Rights Reserved.


These gels are in the  2DWG meta-database. You
can get  a
phosphoprotein gel subset of the 2DWG.











 Select two gels and go flicker compare 
them:


1. First select a different image from each menu.  Note:
you can only compare different images (not an image against
itself).


2. Then press Go Flicker to generate a Flicker comparison of
this pair of images.  


3.  After the images have finished loading, scroll through each lower
image (in turn) by pressing the Control key and then click on a
region, in the Right or Left image, you want to center in the scroll
window. Set each image to be in about the same place. 


Flicker Image Comparison: Flicker is a Java program for
comparing two images.  See the  Flicker home page for
more information on Flicker.












Canvas Size,


 Use JavaPlugin

 


 
Left Image:


 

Right Image:







Enter any image URL (http:// or ftp://) (right)










 





List of gel images of murine T lymphocytes in this database


  •   G1 phase 32P 59g

  •   G2/M phase 32P 59h

  •   IL4 PhosTyr 68dpy

  •   IL2 PhosTyr 68epy

  •   IL2 32P 48c

  •  IL4 32P 48d

    


    


    

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$Date: 1999/01/18 21:23:28 $ / 
    
hornbeck@home.com for 
(
Peter Hornbeck (Editor), 
Phosphoprotein Databases Inc. (PPDB),
    
lemkin@ncifcrf.gov 
for  P. Lemkin (flicker), 
(LECB,NCI/FCRDC)