Flicker comparison of Yeast DB gel image with image URL

You may Flicker compare a Proteome Inc. YPD or SWISS-2DPAGE yeast clickable gels with a gel you specify from a URL. This may be useful for comparing one of your gels against one of these yeast gels where they have identified proteins.

You can also access YPD or SWISS-2DPAGE data for individual spots by turning on the "Click to access DB" checkbox and then clicking on the image from that database.

Instructions

You to select a YPD or SWISS-2DPAGE map image for the left image and compare it with SWISS-2DPAGE or YPD for the right image. Or you can specify the right image from a URL for a corresponding gel image anywhere from the Internet which will be used with Flicker. Once the images are loaded, you may set the "Click to access DB" checkbox in Flicker. Then you may click on a spot in the database gel image and to obtain information on the corresponding protein. It will get that data from YPD or to SWISS-2DPAGE respectively.

You may wish to increase the image canvas size before Flicker starts. The default works nicely for the 1024x768 SVGA.

NOTE: the YPD gel is oriented 6.7-4.25 pH left to right and the SWISS-2DPAGE gel is oriented 4.0-8.0 pH. Therefore, if you are comparing these two gels, you must horizontally flip one of the two gels. You do this in Flicker by 1) clicking on the image to be flipped, then 2) select Flip Horiz. in the TRANSFORM pull down menu. This will take a while, but will replace the image with the flipped image. If you flip the image, the spots are no longer linked correctly to the associated databases.

Canvas Size,
1. Select first yeast gel image:

2. Enter 2nd gel image:

or Enter URL of gel image URL (http:// or ftp://)
   (eg. http://www.proteome.com/gifs/slide1.gif):
   

List of yeast images

$Date: 1999/09/09 20:58:31 $ P. Lemkin, E-mail: lemkin@ncifcrf.gov
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Acknowledgements: This page was derived from the Proteome Inc. YPD and the SWISS-2DPAGE dynamic maps.