GELLAB II+ is a Windows-based PC software program from Scanalytics, Incorporated (8550 Lee Highway, Suite 400 Fairfax, Virginia 22031-1515 USA). GELLAB-II+ provides all the essential tools for the analysis of two dimensional (2-D) electrophoresis protein gels. GELLAB-II+ is based upon the theoretical approach of the GELLAB-II system developed at the National Cancer Institute by Peter Lemkin. GELLAB-II+ is the result of a CRADA technology transfer agreement between NCI and Scanalytics.
GELLAB II analyzes digitized gel images by automatically identifying and outlining individual protein spots, measuring molecular weight, isoelectric point and density for each spot detected. Gel images are grouped into projects based on specifically defined experimental parameters. GELLAB II+ offers the investigator a means of experimental organization, rigorous quantitative analysis, and databasing capabilities to search for specific relationships within the data set.
Gel images are aligned and matched in order to find paired spots. GELLAB II+ maximizes precise alignment between gels by: (a) overlaying regions of the reference gel onto the sample gel to determine matching areas, (b) defining landmarks as anchor points for aligning and pairing the sample gels with the reference image, and (c) finding the spots that match. Comparative data include (for each spot) molecular weight, isoelectric point, concentration, area, integrated optical density, pixel location, pair identifier, raw density and mean density. Using the database query functions in the GELLAB II+ system, users can screen the data and establish definitive relationships between spot pairs and matched gels in a large multi-gel study. In addition, composite gel images can be assembled from several processed gel images providing a clean and simple pattern comparison for presentation and report generation.
To obtain additional information on GELLAB II+ software, please contact Scanalytics at:
Contact: Len Hook, Ph.D., Product Manager Scanalytics, Incorporated 8550 Lee Highway, Suite 400 Fairfax, Virginia 22031-1515 USA E-mail: lhook@scanalytics.com Phone (703) 208-2230 Fax (703) 208-1960